CSVP 370 Diagnosis
May 17, 2019
本次會議組織病理切片資訊：
http://140.120.114.107/slidecenter.php?id=454
Case 1. CSVP 2019-3031 (108-Ma-HG, KCAPO, C.Y. Ma, C.S. Hung, H.Y. Chen, C.C. Cheng, H.Y. Chen, C.C. Lin, and K.S. Yeh)
Hybrid groupers (Epinephelus fuscoguttatus x Epinephelus lanceolatus), 8-10 cm in average. Loss of appetite and lethargic were noted. The morbidity was 5%, and the mortality was 1%. Enlarged cells in capillaries of secondary lamellae were found microscopically.
Morphological diagnosis:
1. Gills: severe, chronic, locally extensive to diffuse, hemorrhagic, necrotizing, fusion of secondary lamellae and enlarged cells in capillaries.
2. Spleen: enlarged cells were found.
3. Liver: fatty change, severe, chronic, diffuse, necrotizing, with lobular disorganization
PCR:
ISKNV (98%)
Etiological Dx.
Hybrid Groupers Infection with Iridovirus
Case 2. CSVP 2019-3032 ( , ADDC NCYU, W.J. Chem , H.C. Kuo, C.L. Chen, Y.C. Su, M.H. Chang, and D.Y. Lo)
Native chickens, 10-week-old, show clinical signs of coughing, dyspnea, and anorexia since two weeks ago. Daily death number was 200 and the total feeding number was 30,000
Morphological diagnosis:
1. Laryngotracheitis, hemorrhagic, necrotized, lymphocytic, heterophlic, fibrinous, locally-extensive, subcacute, severe with eosinophilic intranuclear inclusion bodies in syncytial cells
2. Bronchopneumonia, catarrhal, lymphohistocytic, multifocal, chronic, severe, with eosinophilic intranuclear inclusion bodies in syncytial cells
3. interstitial bronchopneumonia, necrotized, heterophilic, granulomatous, multi-focal, chronic active, severeLaboratory result:
Lab examined;
Staphylococcus spp; PCR: ILTV (+); IBV (-)
Etiological Dx.
Infectious laryngotracheitis with Staphylococcus spp. in black-feather native chickens
Case 3. CSVP 2019-3033 (NTU2018-2738, NTU GIMCP, T.W. Lee, W.H. Huang, and C.H. Liu)
Canine, 11-years-old, castrated male. A mass on right mandibular body was noted and biopsied 2 weeks ago. The mass grows quickly and X-ray shows bone lysis at that area. Total hemimandibulectomy was done on 10/17 and presented for pathological exam.
Morphological diagnosis:
1. expanding the lamina propria and effacing the teeth and bone is a non-encapsulated neoplasm, which is composed of neoplastic odontogenic epithelium accompanied with wide accumulation of eosinophilic to amorphilic, amorphous, hyaline to waxy, weakly birefringent substance (amyloid) and multifocally-distributed deep basophilic, flecks of mineralization. The neoplastic cells are stellate and reticular shaped and arranged in small islands and short cords.
2. Amyloid-producing odontogenic tumor, mandibulectomy, Rt. mandibular mass
Laboratory result:
Congo Red (+)
Etiological Dx.
Amyloid-Producing Odontogenic Tumor in a Dog
Case 4. CSVP 2019-3034 (NTU2018-3050, NTU GIMCP, Y.H. Chen, W.H. Huang, and C.H. Liu )
Canine, 16-years-old, neutered male. An enlarged subcutaneous mass (10 cm × 5 cm × 5 cm) with ulceration and bleeding had been found on the left side of trunk for 2-3 months. An accidently finding of pulmonary hypertension secondary to heartworms infestation was diagnosed after the preoperative examination. The excisional surgery was performed after cardiovascular medical treatment for three days.
Morphological diagnosis:
1. Encapsulated, Hemorrhage with hemosiderin-laden macrophages
2. Low to high density of spindle cells with whole pattern
3. Moderate to high mitotic figures
4. Evenly distributed mast cells and eosinophils
5. Peripheral nerve sheath tumor, grade II (possibly underestimated), with intratumoral infiltrates of mast cells and eosinophils, part of the cutaneous mass at the left back
IHC: NSE, Neurofilament, S100 (+); SMA, Desmin, Toluidine blue, C-kit (-)
Etiological Dx.
Peripheral Nerve Sheath Tumors (PNST) with Mast Cells Proliferative in a Taiwan Dog
Case 5. CSVP 2019-3035 (D18-1657, VDL CMU and NCHU, C.Y. Ko, K. Pringproa, K. Boonsri, Y.C. Chang, and H.Y. Chiou)
Lion (Panthera leo), 16-years-old, female. She was found dead 2 days after the clinical signs of depression and respiratory distress. At necropsy, there was large amount of dark red fluid in the thoracic cavity along with multiple 2-50mm white firm nodules in thoracic cavity, pleural surface, diaphragm, and pericardium.
Morphological diagnosis:
1. Pleura and Pericardium: Mesothelioma
2. Thoracic cavity: Hemothorax
3. Lung: Pneumonia, interstitial, lymphoplasmacytic, multifocal, moderate, chronic, with anthracosis and subpleural fibrosis.
4. Lymph node: Lymphadenitis, necro-hemorrhagic, multifocal, moderate, acute, with anthracosis
IHC: Pan-CK, VIMENTIN (+); calretinin (-)
Etiological Dx.
Mesothelioma in a female lion
Case 6. CSVP 2019-3036(CM19-0102, NCHU ADDC, L.K. Chang, C.Y. Ko, and M.Y. Chia)
Pig, 9-week-old, showed clinical signs of wasting, panting, yellowish watery diarrhea, and swollen joint. The morbidity and mortality rate were 30% (465/1,550) and 10% (155/1,550), respectively. Amoxicillin and colistin were added in the feed, but still failed to control the disease.
Morphological diagnosis:
1. Lymphadenitis, granulomatous, diffuse, chronic, severe, with lymphoid depletion and multinucleated giant cell infiltration, lymphoid tissue.
2. Interstitial pneumoina, non-suppurative, diffuse, chronic, severe, with type II pneumocyte proliferation, lung.
3. Bronchial pneumonia, suppurative, multifocal, subacute, moderate, lung.
Enterotyphlitis, lymphohistiocytic, diffuse, chronic, moderate, with lymphoid depletion and multinucleated giant cell infiltration, locally extensive ulceration and Balamtidium coli infestation, ilium and cecum.
4. Portal triaditis, lymphoplasmacytic, multifocal, chronic, mild, liver.
5. Tubulointerstitial nephritis, lymphohistiocytic, multifocal, chronic, moderate, kidney.
Laboratory result:
PCV2 (+)
Etiological Dx.