CSVP 369 Diagnosis
Apr 19, 2019
本次會議組織病理切片資訊：
http://140.120.114.107/slidecenter.php?id=449
Case 1. CSVP 2019-3025 (WT18-S3, NTU, W.T. Li)
California sea lion (Zalophus californianus), adult, female. She was skinny, lethargic, and died in transport.
Morphological diagnosis:
1. Peritonitis, necrotizing and pyogranulomatous, multifocal, severe, chronic, with intralesional with numerous intralesional fungal spherules and endospores, etiology consistent with Coccidioides spp.
Etiological Dx.
Disseminated Coccidioidomycosis in a California Sea Lion
Case 2. CSVP 2019-3026 (NTU2018-2900, NTU GIMCP, C.F. Kao and C.H. Liu)
Leopard tortoise (Stigmochelys pardalis), 5-year-old, intact female. The animal showed decreased appetite since Oct. 2018 and was found dead on 2018.11.08 Morphological diagnosis:
Pneumonia, proliferative and necrotizing, diffuse, chronic, severe, with numerous intralesional intranuclear coccidian, mild interstitial fibrosis and faveolar edema, lung
Laboratory result:
PCR positive coccidia
Etiological Dx.
Systemic intranuclear coccidiosis
Case 3. CSVP 2019-3027 (NTU2018-2633, NTU GIMCP, Y.H. Hsieh, W.H. Huang, and C.H. Liu)
Hamster, 2.5-year-old, female. Abdominal enlargement noted since this March. Abdominal X-rays showed fluid and ultrasonography revealed cyst-like structures. Transudate was aspirated. The patient was died on 10/7. Ovarian cyst and dilated uterine were found under autopsy performed.
Morphological diagnosis:
1. Surrounding and partially connected to the fibrous cystic wall is a mass structure composed of atrophied ovarian architecture which extensively effaced and replaced by a poor demarcated and unencapsulated neoplasm
2. The neoplasm is composed of polygonal neoplastic cells arranged in small nests and islands supported by abundant dense fibrous stroma
3. The neoplastic cells have variably distinct cell borders and moderate amounts of foamy or fibrillar eosinophilic cytoplasm. Nuclei are oval to polygonal with inconspicuous chromatin and nucleoli
4. Granulosa cell tumor is the most common sex cord stromal tumor. It consists of irregular accumulations of granulosa cells separated
by a supporting stroma of spindle cells, imparting a distinct resemblance to disorganized attempts at follicle formation.
Etiological Dx.
Granulosa cell tumor in a Hamster
Case 4. CSVP 2019-3028 (107034, NTUCM LAC, Y.T. Tsai, W.P. Chen, and H.W. Chen)
Mouse, FVB, 27-month-old, male. The mouse presented periocular/retrobulbar swelling and lens opacity.
Morphological diagnosis:
1. Harderian gland: Harderian gland adenoma, with Harderian gland cyst, dilation, atrophy, interstitial fibrosis, and interstitial pyogranulomatous inflammation
2. Eye: Lens: Cataract, diffuse, severe, with mineralization and subcapsular fibrosis
3. Retina: Atrophy, diffuse, severe
4. Uvea: Anterior uveitis, subacute, diffuse, moderate, with anterior and posterior synechia, keratolenticular adhesions and ciliary-lenticular adhesions
5. Cornea: Keratitis, subacute, diffuse, slight, with neovascularization and goblet cells metaplasia
6. Vitreous: Vitritis, subacute, locally extensive, slight, with fibrosis
7. Optic nerve: Gliosis and axonal degeneration, focal, slight, with axonal spheroids
IHC: CD31, Factor VIII, CD34 (+)
Hemangiosarcoma, grade 2, in testis
Etiological Dx.
Harderian Gland Adenoma and Cataract in a FVB/N Mouse
Case 5. CSVP 2019-3029 (OT107-030, NPUST, S.H. Kuo, X.L. Wu, and Y.C. Li)
Canine, mongrel, 13-year-old, spayed female. A right lower gingival mass in the premolar area was noted with suspected regional osteolysis of the nearby mandible. Incisional surgical biopsy was then performed on March 30, 2018.
Morphological diagnosis:
1. Microscopically, the unencapsulated lesions consist of neoplastic cells forming irregularly vascular channels which filled with erythrocytes. Multiple thrombi are noticed. There is ulcerative inflammation with infiltration of numerous of neutrophils.
2. neoplastic cells forming irregularly vascular channels which filled with erythrocytes. Multiple thrombi are noticed
3. Markedly filling greater than 80% of the mandibular marrow spaces, disrupting the cortex, and extending into the periosteum, nearby lamina propria, and even gingival mucosa epithelium with severe extensive ulceration, coagulative necrosis, and hemorrhages is an unencapsulated, infiltrative, densely cellular neoplasm. The growth consists of pleomorphic spindle cells arranged in short interlacing streams and whorls upon a fine fibrovascular matrix and frequently embedded within lacy deposits of brightly eosinophilic matrix (resembling osteoid), suggestive of an osteoid-producing nature. The proliferating bony trabeculae are often poorly differentiated with lacuna filled with immature, pleomorphic, polygonal osteocytes containing hyperchromatic nuclei and visible nucleoli.
Etiological Dx.
Gingival hemangiosarcoma in a mongrel dog
Central osteosarcoma, telangiectatic variant, grade III, with ulcerative gingivitis and osteolysis, total hemimandibulectomy, Rt. gingival mass
Case 6. CSVP 2019-3030(CO18-730L, GIVP NCHU, Zhi-Yi Lin, Yen-Chi Chang,
Jiunn-Wang Liao, and Hue-Ying Chiou)
Spiny-tailed iguana (Ctenosaura similis), 1-month-old, was found dead after clinical signs including dehydrated and emaciation. At necropsy, multifocal white nodules were spotted on the surface of heart and liver. The lung was diffusely wet.
Morphological diagnosis:
1. Intraendothelial protozoal organisms, disseminated, severe, chronic, etiology consistent with Haemococcidia spp., liver, lung, heart, and bone marrow
2. Calcification, multifocal to coalescing, severe, acute, myocardium
Laboratory result:
PCR: 97% identity with Lankesterella minima
Etiological Dx.